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KMID : 1161420150180040468
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Journal of Medicinal Food
2015 Volume.18 No. 4 p.468 ~ p.475
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Different Solvent Fractions of Acanthopanax senticosus Harms Exert Antioxidant and Anti-Inflammatory Activities and Inhibit the Human Kv1.3 Channel
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Jiang Yunyao
Wang Myeong-Hyeon
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Abstract
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The potential antioxidant and anti-inflammatory activities of Acanthopanax senticosus Harms were evaluated and its effect on the human Kv1.3 potassium channel was detected. The ethyl acetate fraction possessed the highest phenolic (289.19±7.43?mg tannic acid equivalents/g) and flavonoid (10.80±0.67?mg quercetin equivalents/g) contents and exhibited stronger antioxidant effects than other fractions in most of the antioxidant assays. On the other hand, the dichloromethane (CH2Cl2) fraction showed the strongest anti-inflammatory activity. The CH2Cl2 fraction inhibited the expression of inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-α and interleukin 1β mRNAs, and the generation of reactive oxygen species in lipopolysaccharide-induced RAW 264.7 cells. Also, the peak current was inhibited 54.8%±17% by the CH2Cl2 fraction in voltage-clamp recording from Xenopus laevis oocytes. Our research demonstrated that fractions of A. senticosus have great potential to be a source of edible antioxidant and anti-inflammatory agents.
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KEYWORD
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cytotoxicity, DNA damage, nitric oxide, phenolic content, protein damage, ROS
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